Green Approach for Synthesis of Silver Nanoparticles from Marine Streptomyces- MS 26 and Their Antibiotic Efficacy

Antibiotic resistant pathogens pose an enormous threat to the treatment of various serious infections. To overcome this condition, a periodic replacement of the new and existing antibiotic is necessary. Antibiotics in combination with biosynthesized silver nanoparticles minimize the antibiotic doses to cure the dreaded diseases. In this study, Silver nanoparticles were bio-synthesized by extracellular method using Marine Streptomyces sp., isolated from Muttukadu estuary. It was observed that the aqueous silver ions, when exposed to a filtrate of Streptomyces sp., were reduced in solution, thereby leading to formation of extremely stable silver nanoparticles. These silver nanoparticles were characterized by means of several techniques. The biosynthesized silver nanoparticles were also evaluated for their synergistic effect with twelve different antibiotics against the MTCC pathogens. The UV Spectrophotometer revealed the conformation of silver nanoparticles by yielding silver plasmon absorption maxima at 420 nm and Field emission scanning electron microscopy [SEM] micrograph and Particle size analysis indicates the uniform spherical particles at the size range of 50 to 76 nm. The Fourier Transform Infrared [FTIR] Spectroscopy confirmed the presence of proteins as the stabilizing agent surrounding the silver nanoparticle. Atomic force microscopy [AFM] showed the particle height, average roughness of the particles. In this study the combined formulation of silver nanoparticles from Marine Streptomyces species MS-26 with twelve standard antibiotic discs selected from seven groups having a different mode of action was found to be enhanced greatly against the MTCC pathogens Micrococcus luteus [1538], Pseudomonas sp.,[129] Staphylococcus aureus[96] and multi drug resistant pathogen.